--- title: The molecular anti-metastatic potential of CBD and THC from Lebanese Cannabis via apoptosis induction and alterations in autophagy description: Discover the promising anti-cancer potential of CBD and THC from Lebanese Cannabis, focusing on their mechanisms in breast cancer cell inhibition and apoptosis induction. image: https://420intel.com/hubfs/AI-Generated%20Media/Images/scientists%20doing%20testing.jpeg --- [![420intel.com](https://420intel.com/hs-fs/hubfs/Asset%2016-1.png?width=300&height=162&name=Asset%2016-1.png "420intel.com")](https://420intel.com) - [Home](https://420intel.com) - [Advertise](https://420intel.com/advertise) - [Directory](https://420intel.com/directory) - [Topics](https://420intel.com/blog) - [Recreational News](https://420intel.com/blog/tag/recreational-cannabis-news) - [Business News](https://420intel.com/tag/cannabis-business-news) - [Politics](https://420intel.com/tag/cannabis-politics) - [Technology](https://420intel.com/tag/cannabis-technology-news) - [Research](https://420intel.com/tag/medical-cannabis-news) - [Contact](https://420intel.com/contact-us) - [Follow us on Facebook](https://www.facebook.com/420intel) - [Follow us on LinkedIn](https://www.linkedin.com/company/420-intel/) - [Follow us on Twitter](https://x.com/420intel) - [Follow us on Instagram](https://www.instagram.com/420intel) ### Press ESC to close [![420intel.com](https://420intel.com/hs-fs/hubfs/Asset%2016-1.png?width=300&height=162&name=Asset%2016-1.png "420intel.com")](https://420intel.com) - [Home](https://420intel.com) - [Advertise](https://420intel.com/advertise) - [Directory](https://420intel.com/directory) - [Topics](https://420intel.com/blog) - [Recreational News](https://420intel.com/blog/tag/recreational-cannabis-news) - [Business News](https://420intel.com/tag/cannabis-business-news) - [Politics](https://420intel.com/tag/cannabis-politics) - [Technology](https://420intel.com/tag/cannabis-technology-news) - [Research](https://420intel.com/tag/medical-cannabis-news) - [Contact](https://420intel.com/contact-us) - [Follow us on Facebook](https://www.facebook.com/420intel) - [Follow us on LinkedIn](https://www.linkedin.com/company/420-intel/) - [Follow us on Twitter](https://x.com/420intel) - [Follow us on Instagram](https://www.instagram.com/420intel) # The molecular anti-metastatic potential of CBD and THC from Lebanese Cannabis via apoptosis induction and alterations in autophagy - [![Buzz](https://420intel.com/hs-fs/hubfs/Buzz-No%20Outline-2.png?width=80&height=80&name=Buzz-No%20Outline-2.png) Buzz](https://420intel.com/blog/author/buzz) - [Medical Cannabis News](https://420intel.com/blog/tag/medical-cannabis-news) - 28 October 2024 The medicinal plant *Cannabis sativa L. (C. sativa)* is currently being extensively studied to determine the full extent of its therapeutic pharmacological potential. Δ9-tetrahydocannabinol (THC) and cannabidiol (CBD) are the most thoroughly investigated compounds. We aimed to explore the anticancer activity of cannabinoids mixture isolated from the Lebanese *C. sativa* plant in ratios comparable to the local medicinal plant, to elucidate its mechanism of action in breast cancer cells in vitro. Cells were subjected to cytotoxicity assay, cell cycle analysis, Annexin V/PI dual staining, cell death ELISA, immunofluorescence, in addition to western blot analysis of apoptotic and autophagy markers. We further evaluated the anti-metastatic effect of cannabinoids on MDA-MB-231 using the scratch wound-healing, trans-well migration and invasion assays. Our results revealed the promising therapeutic benefits of CBD/THC on inhibiting the growth of breast cancer cells by promoting cellular fragmentation, phosphatidylserine translocation to the outer membrane leaflet and DNA fragmentation in both cell lines while inhibiting the motility of the triple negative breast cancer cells. In our study, CBD/THC mixture was found to exhibit a pro-apoptotic activity via the activation of the mitochondrial apoptotic pathway, independent from ROS production while also suggesting the activation of a caspase-dependent apoptotic pathway. Even though autophagy was altered upon exposure to the cannabinoid mixture, our data suggested that it is not the mechanism responsible of inducing cell death. In conclusion, our study demonstrates the promising therapeutic benefits of CBD and THC isolated from the Lebanese *C. sativa* plant on breast cancer cells in vitro*.*   [*by Nature*](https://www.nature.com/articles/s41598-024-76340-x) [Medical Cannabis News](https://420intel.com/blog/tag/medical-cannabis-news) ![Buzz](https://420intel.com/hs-fs/hubfs/Buzz-No%20Outline-2.png?width=200&height=200&name=Buzz-No%20Outline-2.png) #### [Buzz](https://420intel.com/blog/author/buzz) At 420 Intel, we cover cannabis legalization news throughout the world, offer reliable information for cannabis business owners, detail technological advances that impact the marijuana industry, cover marijuana rallies from across the globe, and everything in between. - - - [Recreational Cannabis News](https://420intel.com/blog/tag/recreational-cannabis-news) [![Cancer Action Network opposing bill to allow cannabis smoking lounges](https://420intel.com/hs-fs/hubfs/AI-Generated%20Media/Images/The%20image%20depicts%20a%20vibrant%20rally%20outside%20the%20state%20legislature%20in%20Carson%20City%20filled%20with%20passionate%20advocates%20holding%20colorful%20banners%20and%20signs%20The%20crowd%20is%20diverse%20consisting%20of%20individuals%20of%20various%20ages%20all%20united%20in%20their%20determination%20to%20sup.jpeg?width=550&height=395&name=The%20image%20depicts%20a%20vibrant%20rally%20outside%20the%20state%20legislature%20in%20Carson%20City%20filled%20with%20passionate%20advocates%20holding%20colorful%20banners%20and%20signs%20The%20crowd%20is%20diverse%20consisting%20of%20individuals%20of%20various%20ages%20all%20united%20in%20their%20determination%20to%20sup.jpeg)](https://420intel.com/blog/cancer-action-network-opposing-bill-to-allow-cannabis-smoking-lounges) - [![Buzz](https://420intel.com/hs-fs/hubfs/Buzz-No%20Outline-2.png?width=80&height=80&name=Buzz-No%20Outline-2.png) Buzz](https://420intel.com/blog/author/buzz) - 25 March 2025 #### [Cancer Action Network opposing bill to allow cannabis smoking lounges](https://420intel.com/blog/cancer-action-network-opposing-bill-to-allow-cannabis-smoking-lounges) ### Advocates Push for Cancer Legislation in Carson City Today in Carson City, cancer advocates... [Cannabis Business News](https://420intel.com/blog/tag/cannabis-business-news) [![Verano Holdings Corp. Set to Begin “Save the Bits” Campaign for Lynn Sage Breast Cancer Foundation](https://420intel.com/hs-fs/hubfs/Imported_Blog_Media/AdobeStock_367533788.jpeg?width=550&height=395&name=AdobeStock_367533788.jpeg)](https://420intel.com/blog/verano-holdings-corp-set-begin-save-bits-campaign-lynn-sage-breast-cancer) - [![Buzz](https://420intel.com/hs-fs/hubfs/Buzz-No%20Outline-2.png?width=80&height=80&name=Buzz-No%20Outline-2.png) Buzz](https://420intel.com/blog/author/buzz) - 26 September 2024 #### [Verano Holdings Corp. Set to Begin “Save the Bits” Campaign for Lynn Sage Breast Cancer Foundation](https://420intel.com/blog/verano-holdings-corp-set-begin-save-bits-campaign-lynn-sage-breast-cancer) *Multi-state cannabis company, Verano Holdings Corp., started a fundraising campaign with their...* [Medical Cannabis News](https://420intel.com/blog/tag/medical-cannabis-news) [![Medical Marijuana might help Cancer pain](https://420intel.com/hs-fs/hubfs/Imported_Blog_Media/Medical%20Marijuana%20might%20help%20Cancer%20pain.jpg?width=550&height=395&name=Medical%20Marijuana%20might%20help%20Cancer%20pain.jpg)](https://420intel.com/blog/medical-marijuana-might-help-cancer-pain) - [Admin](https://420intel.com/blog/author/admin) - 7 March 2024 #### [Medical Marijuana might help Cancer pain](https://420intel.com/blog/medical-marijuana-might-help-cancer-pain) Cannabis research powerhouse Israel produces more cancer and medical marijuana data. Cannabis can... - [Follow us on Facebook](https://www.facebook.com/420intel) - [Follow us on LinkedIn](https://www.linkedin.com/420intel) - [Follow us on Twitter](https://twitter.com/420intel) - [Follow us on Instagram](https://www.instagram.com/420intel) Back to Top ```json { "@context" : "https://schema.org", "@type" : "BlogPosting", "author" : { "@type" : "Person", "name" : "Buzz", "url" : "https://420intel.com/blog/author/buzz" }, "dateModified" : "2024-10-28T15:11:58.997Z", "datePublished" : "2024-10-28T15:11:58.000Z", "headline" : "The molecular anti-metastatic potential of CBD and THC from Lebanese Cannabis via apoptosis induction and alterations in autophagy", "image" : [ "https://420intel.com/hubfs/AI-Generated%20Media/Images/scientists%20doing%20testing.jpeg" ], "mainEntityOfPage" : { "@id" : "https://420intel.com/blog/the-molecular-anti-metastatic-potential-of-cbd-and-thc-from-lebanese-cannabis-via-apoptosis-induction-and-alterations-in-autophagy", "@type" : "WebPage" }, "publisher" : { "@type" : "Organization", "logo" : { "@type" : "ImageObject", "url" : "https://420intel.com/hubfs/Asset%2016.png" }, "name" : "420 Intel" } } ```